Journal: International Journal of Molecular Sciences

Article Title: Peptide-Guided Photodynamic Therapy via Integrin αvβ6 in Pancreatic Cancer

doi: 10.3390/ijms27041838

Figure Lengend Snippet: ( a ) Expression of αvβ6 and αvβ3 integrins in pancreatic cancer cell lines. ( a ) Normalized fluorescence measurements showing integrin expression levels in BxPc3, MiaPaca2, and Panc1 cells. BxPc3 cells display significantly higher αvβ6 expression compared with MiaPaca2 and Panc1, whereas αvβ3 expression is detectable at lower levels with a different expression profile across cell lines. Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by post hoc multiple comparison testing; differences were considered statistically significant at *** p < 0.001 (* p < 0.05 where indicated). ( b ) Western blot results showing β6 integrin expression levels normalized to protein concentration (pg β/µg protein) across different PDAC cell lines. BxPc3 expresses high levels of β6 (1411 pg/µg), MiaPaca2 and Panc1 expresses show no detectable levels of this integrin.

Article Snippet: Primary antibodies included Anti-Integrin αvβ3 (Integrin β3 (D7X3P) XP ® Rabbit mAb, Cell Signaling) at a 1:500 dilution and Anti-Integrin αvβ6 (clone EM05201, ZooMAb ® Rabbit Monoclonal ZRB1104) at a 1:1000 dilution, incubated overnight at 4 °C.

Techniques: Expressing, Fluorescence, Comparison, Western Blot, Protein Concentration